DETERMINATION OF ACRAB, TOLC EFFLUX PUMPS GENES IN STRONG BIOFILM FORMER KLEBSIELLA PNEUMONIAE

Abstract

Klebsiella pneumoniae (K. pneumoniae) is a gram-negative bacilli, encapsulated, facultative anaerobic, lactose-fermenting, non-mobile, rod- shaped bacterium, they do have a high tendency to become antibiotic resistant. Aims of study: Detection of acrAB, tolC efflux pumps genes in strong biofilm of K. pneumoniae. Methods: Micro-titer plate (MtP) quantitative method used to determine the biofilm production. The phenotypic and qualitative finding of the efflux pump was achieved by Ethidium Bromide-agar Cart Wheel Method, All isolates were screened for their antibiotic resistance against a selected (35) clinically antibiotics by VITEK MIC method. Results: K. pneumoniae was mainly isolated from urine samples 52% followed by sputum 16%, swap 14%, body fluids 10%, bedsore 5% and folly's catheters were 3%, the distribution of K. pneumoniae isolates according to sex showed that 55% occurs in males and 45% of isolates were from females. Most of the K. pneumoniae isolates were collected from patients with age group ranged from (1 to 80 years). All of isolates were biofilm producers, with (43%) isolates as strong, (32%) isolate as moderate and (25%) isolates as weak biofilm producers. DNA has been extracted from (51 isolates) that are only strong biofilm and positive efflux pumps. Highly EP founded in the bacteria with different concentration of EtBr 85 (85.00%), 91 (91.00%), 94 (94.00%), and 94 (94.00%) with EtBr (0, 0.5, 1, 1.5, 2 mg/l) respectively. K.pneumoniae is found to be resistant to many antibiotics MDR 33% and XDR 67%. The results of efflux pumps genes were acrAB (72.5%) and tolC was (43%).

Keywords: K. pneumoniae; biofilm formation; Phenotypic; genotypic; acrAB; tolC.