THE EFFECT OF THYROID PEROXIDASE (TPO) GENE POLYMORPHISM SNP RS732609 ON THE THYROID-STIMULATING HORMONE AND ANTI-TPO ANTIBODY IN A SAMPLE OF IRAQI PATIENTS WITH HYPOTHYROIDISMS DISORDER.

Abstract

              Background & Objective : The purpose of this study was to look at the relationship between the level of severity of the medical condition and SNPs in the TPO gene (rs732609), anti-TPO stages, and TSH levels of Iraqi patients suffering from auto immune hypothyroidism.  

Method: Blood samples were taken from 50 a hypothyroid patient as well as fifty people in good health with the same condition in order to look for DNA. The SNP rs732609 was subsequently identified using the RT-PCR-HRM (High Resolution Melting) technique with the latest version of Eva green the pigment . Also, Chemical analyses were carried out on the Cobus E411 device, which uses electro chemiluminescence (ECL) technology to transform electrical energy into light (radiative energy), for determining the amounts of Thyroid stimulating hormone  and anti-TPO antibodies.

Result: Regarding the TPO rs732609 A/C polymorphism, patients with hypothyroidism had significantly higher CC genotypes of rs732609 A/C and the C allele; the p-value for the (AA+AC) CC genotype is 0.0001, the odd ratio (95% CI) is 16.9, and the level of TSH was significantly higher than expected at p-value (0.05), and the level of anti-TPO ab in serum was also significantly higher at p-value (0.04).

Conclusion: Our findings show a link among anti-TPO antibodies levels along with a variety of genotypes in hypothyroid people, as well as a link between the rs732609A/C variations and auto immune hypothyroidism. We also found a link among the severeness of the condition and the rs732609A/C polymorphisms.

Keywords: Hypothyroidism, thyroid peroxidase gene, single nucleotide polymorphism, Anti -Tpo.