SYNTHESIS, CHARACTERIZATION, AND ANTIMICROBIAL ACTIVITY ASSESSMENT OF NOVEL PEPTIDE ISOSTERES

Authors

  • Shakti, Vinay Kumar Gupta, Anil Kumar, Jyoti Prakash Guru, Shakeel Ahmad, Debjani Sarkar, Kavita, Vikram, Rinchi Bora Author

Abstract

In this study, we investigated potential new peptide isostere compounds as potent antimicrobial agents. We synthesized these compounds, characterized them, and confirmed their purity using various techniques such as NMR spectroscopy, MS, and HPLC. We determined their antimicrobial efficacy by measuring the minimum inhibitory concentrations (MICs) and the minimum bactericidal/fungicidal concentrations (MBCs/MFCs). We also checked if they were harmful to mammalian cells using a special test. Some peptide isosteres worked better against germs than others. We found that MICs from 6.25 to 50 mg/mL and MBC/MFC values ranged from 12.5 to over 50 mg/mL. In addition, these compounds demonstrated minimal cytotoxicity towards mammals and selective activity against microorganisms. It has been found that peptide isosteres may have therapeutic potential based on a study of their structure-activity relationship. This research aims to address the challenge of antibiotic resistance through the development of targeted antimicrobial treatments through peptide-based therapeutics. By synthesizing peptide isosteres, alternative treatment options for microbial infections could be developed, current antimicrobial agents could be enhanced, and peptide-based research could be advanced. A study on peptide isosteres has demonstrated their efficacy as powerful antimicrobial agents, enabling the development of new therapeutic strategies.

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Published

2024-07-06

How to Cite

SYNTHESIS, CHARACTERIZATION, AND ANTIMICROBIAL ACTIVITY ASSESSMENT OF NOVEL PEPTIDE ISOSTERES. (2024). Romanian Journal of Diabetes, Nutrition and Metabolic Diseases, 31(2). https://jrdiabet.ro/index.php/RJDNMD/article/view/379