MOLECULAR DETECTION OF TRICHOMONAS GALLINAE INFECTION OF LOCAL PIGEONS (COLUMBA LIVIA DOMESTICA) IN AL-QADISIYAH PROVINCE, IRAQ

Abstract

Trichomonas gallinae isolates from local pigeons in Al-Diwaniya province, Iraq were amplified by polymerase chain reaction using ITS1- 5.8S and Fe hydrogenase gene and characterized by molecular tools to determine the geographical location and the sequences were analyzed phylogenetically. The isolates in the present study were 100% similar to T. gallinae isolates of Iran and Austria. Random samples found positive for trichomoniasis in culture and throat swab in live birds were confirmed by PCR. The positive samples showed clear band at 370 and 528 bp for ITS1- 5.8S and Fe hydrogenase gene respectively.

Keywords: Trichomonas Gallinae, Pigeons, PCR, Fe hydrogenase and ITS1- 5.8S