MOLECULAR IDENTIFICATION OF FASCIOLA SPP IN CATTLE IN AL-DIWANYIA, IRAQ

Abstract

 The reading focused on taking isolates of cow liver from the butchers’ market in the city of Diwaniyah for the period from Agest 2022 to April 2023 in order to diagnose flatworm infection, specifically genus Fasciola, which relied on molecular diagnosis as an polymerase chain reaction (PCR) for the specific identification of the parasite and based on the 16s RNA genetic region as a conservative region with a size of (433 bp). According to previous studies, which came after electrophoresis and showing the phylogenetic relationship with isolates recorded from Egypt, China, and America on the NCBI website, the results showed a high percentage of identity with the Fasciola gigntica species and similarity reaching from (98.7-99.16%).

Keywords: Fasciola gegntica,Molecular, flatworm,phylogenetic, polymerase