EFFECTIVE ANTIFUNGAL COMPOUNDS OF LACTOBACILLUS CASEI AGAINST VULVOVAGINAL CANDIDIASIS FOR WOMEN IN THI-QAR GOVERNORATE

Abstract

One hundred fifty four samples were collected from Bint Al-Huda Teaching Hospital in Thi- Qar divided to 104 women infected urinary tract infection (UTI) and 50 healthy women as control group, the result revealed that 48 patient infected with vulvovaginal candidiasis, L.casei (ATCC39392)   extracted  and chemical compounds identified by Gas Chromatography, the extract of L. casei inhibited C. albicans  in well diffusion assay and the inhibition  zone, its was 15 mm, while minimum inhibiter concentration  was 32 μg/ml  , examine effected of extract as biofilm showed that 20.8% of isolates formed strong biofilm while moderate and weak 70.8% and 8.3% respectively, the extracted have significant effect as antibiofilm, it was able to reduce biofilm strongly 60% , moderate 20% while there was  20% not effected, examination cytotoxicity of this extract showed that there is low toxicity on normal cells of dermal cell line and hepatic cell line, Ic50 was and 192.7µs/ml for both WRL-68 and HdFn cell lines, which inhibition rate 14.2 and 16.1 for HdFn and WRL-68 respectively  at concentration 400 µg /ml.                                                                                                            

Key words:  Vulvovaginal candidiasis, Lactobacillus casei , Probiotics, Antifungal