TYPES OF ASPERGILLUS FUNGI ISOLATED FROM THE AIR OF THE AREA BETWEEN THE TWO HOLY MOSQUES IN HOLY KARBALA

Abstract

The study included molecular diagnosis of some types of Aspergillus fungi in specific areas between the two holy mosques of the city of Karbala. With the aim of identifying the types of Aspergillus present in the region and their importance to community health. Samples were taken from the atmospheric air using an air-sampler system. Using the technique of random polymorphic DNA replication (genetic fingerprinting) after the DNA was extracted from each isolate at a final concentration of pb (524) per 2-3 grams of wet mycelium with 100% purity, using primers ITS1 and ITS4, and by electrophoresis through a gel. Agarose with a concentration of 1.5%, dyed with ethidium bromide, and photographed under ultraviolet light. The genetic fingerprint of four isolates isolated from the air was determined by the appearance of DNA duplication bands for each isolate with one or more primers, and they included: 1. Aspergillus fumigatus, A.fumigatus 2, A. terreus were registered in the Global GenBank with serial numbers (OR492482. OR492612. OR497754) respectively, with a similarity rate of 100% with global fungi.

Keyword: Fungi Molecular diagnosis of fungi, Aspergillus spp.