EFFECT OF GLYCYRRHIZA GLABRA L. ROOT EXTRACT ON CANDIDA SPP.

Abstract

The current study aimed to investigate the active compounds in the roots of Glycyrrhiza glabra L. by using high-performance liquid chromatography (HPLC).Ten effective compounds were separated from the licorice roots, with glycyrrhizin being the most concentrated compound. An alcoholic extract of licorice roots was prepared to assess its antagonistic activity against various Candida species. The study found that licorice inhibited the growth of all tested Candida strains. Additionally, a nano-material was synthesized from licorice roots in the form of silver nitrate. The results confirmed shifts in chemical group frequencies toward infrared frequencies (FTIR) and revealed new levels in the high and low regions of X-ray diffraction (XRD). Scanning electron microscopy (SEM) showed relatively uniform shapes for the hybrid nano compound. Energy-dispersive X-ray spectroscopy (EDS) indicated the presence of silver, carbon, and chlorine in nearly spherical forms. Overall, this prepared hybrid nano compound exhibited higher inhibitory efficacy compared to the raw extract, effectively suppressing all Candida species across various concentrations (500, 400, 300, 200, and 100 micrograms/mL) using the agar well diffusion method. In conclusion, the alcoholic extract of licorice roots contains terpenoid saponins. The superiority of the alcoholic extract of licorice roots in inhibiting Candida species, it is possible to prepare a nano-hybrid extract from licorice roots. Efficiency of the nano-hybrid extract in inhibiting all types of Candida at all concentrations. The alcoholic extract of the licorice plant excels in scavenging free radicals.

Keywords: Licorice roots, Silver nanoparticles, X-ray, FTIR, XRD, SEM, EDS.